Maintenance history: rpc00053

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2015-11-13 Depositor   Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7 25 light (5000 lux)   14     20 flask (100-mL)
2015-11-13 Datasheet   Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7 25 light (5000 lux)   14     60 flask (200-mL)
2015-11-13     Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7 25 light (5000 lux)   14       flask (200-mL)
2017-10-01     Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7 24 light (PPFD 55–60 µmol m-2 s-1)   28   3–5 callus (diameter 5–7 mm) 60 flask (200-mL)
2019-02-07 Present   Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7 24–25 light (PPFD 55–60 µmol m-2 s-1)   28   3–5 callus (diameter 5–7 mm) 60 flask (200-mL)