Maintenance history: rpc00017
Date |
Description |
Past availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|
2002-04-01 |
RIKEN Cell Bank |
modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 |
25–27 |
dark |
140 |
7 |
3.5 |
packed cells |
40 |
flask (100-mL) |
|
2003-05-14 |
Release |
modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 |
25–27 |
dark |
140 |
7 |
3.5 |
packed cells |
40 |
flask (100-mL) |
|
2014-11-07 |
modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 |
27 |
dark |
140 |
7 |
15 |
40 |
flask (100-mL) |
|||
2017-10-01 |
modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 |
27 |
dark |
140 |
7 |
15 |
see the protocol |
25 |
flask (100-mL) |
||
2019-02-07 |
modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 |
27 |
dark |
140 |
7 |
15 |
see the protocol |
25 |
flask (100-mL) |
||
2023-07-07 |
Present |
modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, 1.2% (w/v) agar, pH 6.5 |
27 |
dark |
28–56 |
4-7 callus (diameter 3-5 mm) |
40 |
flask (100-mL) |