Maintenance history: rpc00035

rpc00035: Nicotiana tabacum Xan-1 callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2005-12-08	Depositor	Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, pH 5.7	27	dark	130	7	5–8		90	Erlenmeyer flask (300-mL)
2005-12-08	Datasheet	Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.8% (w/v) agar, pH 5.7	27	dark		28			80	Erlenmeyer flask (300-mL)
2006-10-26	Release	Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (200-mL)
2017-10-01		Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 3 mm)	80	Erlenmeyer flask (200-mL)
2019-02-07		Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 3 mm)	80	Erlenmeyer flask (200-mL)
2023-11-01	Present	Living cells	Available	Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 3 mm)	40	Erlenmeyer flask (100-mL)