Maintenance history: rpc00035

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2006-10-26 Depositor   Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, pH 5.7 27 dark 130 7 5–8   90 flask (300-mL)
2006-10-26 Datasheet   Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.8% (w/v) agar, pH 5.7 27 dark   28     80 flask (300-mL)
2006-10-26     Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7 27 dark   28       flask (300-mL)
2014-11-07     Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7 27 dark   28       flask (200-mL)
2017-10-01     Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7 27 dark   28   3 callus (diameter 3 mm) 80 flask (200-mL)
2019-02-07 Present   Gamborgʼs B5 medium, 1 µM 2,4-D, 0.04 µM kinetin, 0.9% (w/v) agar, pH 5.7 27 dark   28   3 callus (diameter 3 mm) 80 flask (200-mL)