Maintenance history: rpc00009

rpc00009: Nicotiana tabacum T-13 cell suspension culture

Date	Description	Past availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank		Murashige and Skoog medium, pH 5.8	25–27	dark	120	7	10		75	Erlenmeyer flask (300-mL)
2003-05-14	Release		Murashige and Skoog medium, pH 5.8	25–27	dark	120	7	10		75	Erlenmeyer flask (300-mL)
2005-06-01		distribution suspended	Murashige and Skoog medium, pH 5.6	27	dark	120	7	10	settled cells	75	Erlenmeyer flask (300-mL)
2014-11-07		distribution suspended	Murashige and Skoog medium, pH 5.6	27	dark	120	7	10	settled cells	75	Erlenmeyer flask (300-mL)
2015-09-01		distribution suspended	Murashige and Skoog medium, pH 5.8	27	dark	120	7	10	settled cells	75	Erlenmeyer flask (300-mL)
2017-10-01			Murashige and Skoog medium, pH 5.8	27	dark	120	7	10	settled cells	75	Erlenmeyer flask (300-mL)
2019-02-07			Murashige and Skoog medium, pH 5.8	27	dark	120	7	10	settled cells	75	Erlenmeyer flask (300-mL)
2023-07-10			Murashige and Skoog medium, pH 5.8	27	dark	120	7	8		75	Erlenmeyer flask (300-mL)
2023-11-01	Present		Murashige and Skoog medium, pH 5.8	27	dark	120	7	8		75	Erlenmeyer flask (300-mL)