Maintenance history: rpc00057

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2018-03-23 Depositor   Murashige and Skoog medium, 1 mg/L 2,4-D, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 25 dark   30–60        
2018-03-23     Murashige and Skoog medium, 1 mg/L 2,4-D, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 27 dark   28   3–4 callus (diameter 3–5 mm) 40 flask (100-mL)
2019-02-07 Present   Murashige and Skoog medium, 1 mg/L 2,4-D, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 27 dark   28   3–4 callus (diameter 3–5 mm) 40 flask (100-mL)