Maintenance history: rpc00040

rpc00040: Nicotiana tabacum GF11 transgenic cell suspension culture

Date	Description	Past availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2008-07-18	Depositor		modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.5		95
2008-07-18	Datasheet		modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.5		95	Erlenmeyer flask (300-mL)
2011-09-16	Release		modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.5		95	Erlenmeyer flask (300-mL)
2014-11-07			modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2–2.4		95	Erlenmeyer flask (300-mL)
2017-10-01			modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2–2.4		95	Erlenmeyer flask (300-mL)
2019-02-07			modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	3–3.2		95	Erlenmeyer flask (300-mL)
2023-11-01	Present		modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	3–3.2		95	Erlenmeyer flask (300-mL)