Maintenance history: rpc00043

rpc00043: Nicotiana tabacum 3n-3 callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2006-03-23	Depositor	Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	26	dark
2006-03-23	Datasheet	Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	27	dark		28			80	Erlenmeyer flask (300-mL)
2013-02-28	Release	Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (200-mL)
2017-10-01		Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	27	dark		28		3-5 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)
2019-02-07		Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	27	dark		28		3-5 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)
2023-11-01	Present	Living cells	Available	Murashige and Skoog medium, 0.9% (w/v) agar, pH 5.7	27	dark		28		3-5 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)