Maintenance history: rpc00004
Date |
Description |
Past availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|
2002-04-01 |
RIKEN Cell Bank |
modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, pH 6.1 |
27 |
dark |
120 |
7 |
10 |
72 |
Erlenmeyer flask (300-mL) |
||
2003-05-14 |
Release |
modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 |
27 |
dark |
28 |
Erlenmeyer flask (300-mL) |
|||||
2014-11-07 |
modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 |
27 |
dark |
56 |
100 |
Erlenmeyer flask (300-mL) |
|||||
2017-10-01 |
modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 |
27 |
dark |
56 |
1-2 callus (diameter 8 mm) |
100 |
Erlenmeyer flask (300-mL) |
||||
2019-02-07 |
modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 |
27 |
dark |
56 |
1-2 callus (diameter 8 mm) |
100 |
Erlenmeyer flask (300-mL) |
||||
2023-11-01 |
Present |
modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 |
27 |
dark |
56 |
1-2 callus (diameter 8 mm) |
100 |
Erlenmeyer flask (300-mL) |