Maintenance history: rpc00014

rpc00014: Catharanthus roseus CRA cell suspension culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	25–27	dark	120	7	7		95	Erlenmeyer flask (300-mL)
2003-05-14	Release	Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	25–27	dark	120	7	7		95	Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	27	dark	120	7	7		95	Erlenmeyer flask (300-mL)
2017-10-01		Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	27	dark	120	7	7		95	Erlenmeyer flask (300-mL)
2019-02-07		Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	27	dark	120	7	7		95	Erlenmeyer flask (300-mL)
2023-11-01		Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	27	dark	120	7	4.2		95	Erlenmeyer flask (300-mL)
2024-01-25	Present	Living cells	Available	Murashige and Skoog medium, 0.5 mg/L 2,4-D, pH 6.2	27	dark	120	7	4–5		95	Erlenmeyer flask (300-mL)