Maintenance history: rpc00110
Date |
Description |
Cell status |
Previous availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
2012-03-27 |
Depositor |
Living cells |
Available |
modified Gamborgʼs B5 medium, 0.54 mg/L NAA, 0.2 mg/L kinetin, pH 5.7 |
28 |
dark |
100 |
7 |
3 |
30 |
Erlenmeyer flask (100-mL) |
|
Induction |
Living cells |
Available |
BSS medium, 0.54 µM NAA, 0.2 mg/L kinetin, 0.3% (w/v) gellan gum, pH 5.7 |
27 |
dark |
21–28 |
7 callus (diameter 5 mm) |
30 |
Petri dish (9-cm) |
|||
2024-03-13 |
Present |
Living cells |
Available |
BSS medium, 0.54 µM NAA, 0.2 mg/L kinetin, 0.3% (w/v) gellan gum, pH 5.7 |
27 |
dark |
21–28 |
7 callus (diameter 5 mm) |
30 |
Petri dish (9-cm) |