Maintenance history: rpc00010

rpc00010: Asparagus officinalis Asp-86 callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, pH 5.7	27	dark	120	14	10		90	Erlenmeyer flask (300-mL)
2003-05-14	Release	Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (200-mL)
2006-05-24	Present	Living cells	Not available	Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (200-mL)