Maintenance history: rpc00065

Date

Description

Past availability

Medium

Temperature (°C)

Light condition

Rotation (rpm)

Subculture (days)

Transfer volume (mL)

Transfer method

Medium volume (mL)

Container

2013-02-21

Depositor

Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7

25

light (5000 lux)

14

20

Erlenmeyer flask (100-mL)

2021-03-15

Release

Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7

25

light (PPFD 45-50 µmol m-2 s-1)

28

3-5 callus (diameter 3-10 mm)

60

Erlenmeyer flask (200-mL)

2023-11-01

Present

Murashige and Skoog medium, 0.25% (w/v) gellan gum, pH 5.7

25

light (PPFD 45-50 µmol m-2 s-1)

28

3-5 callus (diameter 3-10 mm)

60

Erlenmeyer flask (200-mL)