Maintenance history: rpc00037

rpc00037: Lithospermum erythrorhizon M18-1 callus culture

Date	Description	Past availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2006-02-08	Depositor		Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	25	dark		30–31				Petri dish (9-cm)
2006-02-08	Datasheet		Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	25	dark		28			30	Petri dish (9-cm)
2007-09-03	Release		Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	25	dark		28				Petri dish (9-cm)
2014-11-07			Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	27	dark		28				Erlenmeyer flask (100-mL)
2017-10-01			Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	27	dark		28		3-5 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)
2018-01-12			Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	23	dark		28		3-5 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)
2018-01-29			Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	23	dark		28		7-9 callus (diameter 3-5 mm)	40	Erlenmeyer flask (100-mL)
2019-02-07			Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	23	dark		28–42		7-9 callus (diameter 3-5 mm)	40	Erlenmeyer flask (100-mL)
2023-11-01	Present		Linsmaier and Skoog medium, 1 µM IAA, 10 µM kinetin, 1% (w/v) agar, pH 6.3	23	dark		28–42		7-9 callus (diameter 3-5 mm)	30	Erlenmeyer flask (100-mL)