Maintenance history: rpc00032

rpc00032: Lotus japonicus Lj cell suspension culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2005-03-17	Depositor	Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	25	dark	100	7	15		80	Erlenmeyer flask (300-mL)
2005-03-17	Datasheet	Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	100	7	15		80	Erlenmeyer flask (300-mL)
2005-10-17	Release	Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	100	7	15		80	Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	100	7	15	settled cells	80	Erlenmeyer flask (300-mL)
2017-10-01		Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	100	7	15	settled cells	80	Erlenmeyer flask (300-mL)
2019-02-07		Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	120	7	10	settled cells	80	Erlenmeyer flask (300-mL)
2023-04-24		Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	120	7	25	settled cells	80	Erlenmeyer flask (300-mL)
2023-11-01		Living cells	Available	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	120	7	25	settled cells	80	Erlenmeyer flask (300-mL)
2024-04-17	Present	Living cells	Preparing	Murashige and Skoog medium, 1 mg/L 2,4-D, pH 5.7	27	dark	120	7	25	settled cells	80	Erlenmeyer flask (300-mL)