Maintenance history: rpc00012

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2003-05-14 RIKEN Cell Bank   Linsmaier and Skoog medium, 0.1 µM 2,4-D, pH 5.7 27 dark 120 7 7   90 flask (300-mL)
2003-05-14     Linsmaier and Skoog medium, 0.1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   28       flask (200-mL)
2014-11-07     Linsmaier and Skoog medium, 0.1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   28–42     80 flask (200-mL)
2016-01-16   distribution suspended Linsmaier and Skoog medium, 0.2 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   56–84     40 flask (100-mL)
2017-10-01   distribution suspended Linsmaier and Skoog medium, 0.2 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   56–84   3 callus (diameter 10 mm) 40 flask (100-mL)
2018-01-12     Linsmaier and Skoog medium, 0.2 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   42–56   3 callus (diameter 10 mm) 40 flask (100-mL)
2019-02-07 Present   Linsmaier and Skoog medium, 0.2 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   28–42   3 callus (diameter 10 mm) 40 flask (100-mL)