Maintenance history: rpc00033

rpc00033: Lotus japonicus LjA callus culture

Date	Description	Past availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2005-03-17	Depositor		Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	25	dark		30–31
2005-03-17	Datasheet		Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28			40	Erlenmeyer flask (100-mL)
2005-10-17	Release		Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (100-mL)
2014-11-07			Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (100-mL)
2017-10-01			Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)
2019-02-07			Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)
2023-11-01	Present		Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)