Maintenance history: rpc00011

rpc00011: Citrullus battich Cba-1 callus culture

Date	Description	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Linsmaier and Skoog medium, 1 µM 2,4-D, pH 5.7	27	dark	120	7	10		90	Erlenmeyer flask (300-mL)
2003-05-14	Release	Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (200-mL)
2014-11-07		Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28			80	Erlenmeyer flask (200-mL)
2017-10-01		Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 8 mm)	80	Erlenmeyer flask (200-mL)
2019-02-07		Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 8 mm)	80	Erlenmeyer flask (200-mL)
2023-11-01	Present	Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 8 mm)	80	Erlenmeyer flask (200-mL)