Maintenance history: rpc00018

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2003-05-14 RIKEN Cell Bank   modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 25–27 dark 140 7 4 packed cells 40 flask (100-mL)
2003-05-14     modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 25–27 dark 140 7 4 packed cells 40 flask (100-mL)
2014-11-07     modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 27 dark 140 7 15   40 flask (100-mL)
2017-10-01     modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 27 dark 140 7 15 see the protocol 25 flask (100-mL)
2019-02-07 Present   modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5 27 dark 140 7 15 see the protocol 25 flask (100-mL)