Maintenance history: rpc00018

Date

Description

Past availability

Medium

Temperature (°C)

Light condition

Rotation (rpm)

Subculture (days)

Transfer volume (mL)

Transfer method

Medium volume (mL)

Container

2002-04-01

RIKEN Cell Bank

modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5

25–27

dark

140

7

4

packed cells

40

Erlenmeyer flask (100-mL)

2003-05-14

Release

modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5

25–27

dark

140

7

4

packed cells

40

Erlenmeyer flask (100-mL)

2014-11-07

modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5

27

dark

140

7

15

40

Erlenmeyer flask (100-mL)

2017-10-01

modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5

27

dark

140

7

15

see the protocol

25

Erlenmeyer flask (100-mL)

2019-02-07

modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5

27

dark

140

7

15

see the protocol

25

Erlenmeyer flask (100-mL)

2023-11-01

Present

modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5

27

dark

140

7

12

see the protocol

25

Erlenmeyer flask (100-mL)