Maintenance history: rpc00018

rpc00018: Spinacia oleracea Spi-12F cell suspension culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5	25–27	dark	140	7	4	packed cells	40	Erlenmeyer flask (100-mL)
2003-05-14	Release	Living cells	Available	modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5	25–27	dark	140	7	4	packed cells	40	Erlenmeyer flask (100-mL)
2014-11-07		Living cells	Available	modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5	27	dark	140	7	15		40	Erlenmeyer flask (100-mL)
2017-10-01		Living cells	Available	modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5	27	dark	140	7	15	see the protocol	25	Erlenmeyer flask (100-mL)
2019-02-07		Living cells	Available	modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5	27	dark	140	7	15	see the protocol	25	Erlenmeyer flask (100-mL)
2023-11-01	Present	Living cells	Available	modified Murashige and Skoog medium, 0.5 mg/L NAA, 1 mg/L BAP, pH 6.5	27	dark	140	7	12	see the protocol	25	Erlenmeyer flask (100-mL)