Maintenance history: rpc00067
Date |
Description |
Past availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|
2013-02-21 |
Depositor |
modified Gamborgʼs B5 medium, 0.25% (w/v) gellan gum, pH 5.7 |
25 |
light (5000 lux) |
14 |
20 |
Erlenmeyer flask (100-mL) |
||||
2021-03-15 |
Release |
modified Gamborgʼs B5 medium, 0.25% (w/v) gellan gum, pH 5.7 |
25 |
light (PPFD 35-40 µmol m-2 s-1) |
28 |
3-5 callus (diameter 3-10 mm) |
60 |
Erlenmeyer flask (200-mL) |
|||
2023-11-01 |
Present |
modified Gamborgʼs B5 medium, 0.25% (w/v) gellan gum, pH 5.7 |
25 |
light (PPFD 35-40 µmol m-2 s-1) |
28 |
3-5 callus (diameter 3-10 mm) |
60 |
Erlenmeyer flask (200-mL) |