Maintenance history: rpc00021

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2003-05-14 RIKEN Cell Bank   Linsmaier and Skoog medium, 1 µM 2,4-D, pH 5.7 27 dark 120 28 10   90 flask (300-mL)
2003-05-14     Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   56       flask (100-mL)
2014-11-07     Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   56       flask (200-mL)
2017-10-01     Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   56   3 callus (diameter 8 mm) 80 flask (200-mL)
2019-02-07 Present   Linsmaier and Skoog medium, 1 µM 2,4-D, 1.2% (w/v) agar, pH 5.7 27 dark   56   3 callus (diameter 8 mm) 80 flask (200-mL)