Maintenance history: rpc00002

rpc00002: Daucus carota kurodagosun cell suspension culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	modified Lin and Staba medium, 0.5 µM 2,4-D, pH 5.7	25–27	dark	130	7	10–20		80	Erlenmeyer flask (300-mL)
2003-05-14	Release	Living cells	Available	modified Lin and Staba medium, 0.2 mg/L 2,4-D, pH 5.7	25–27	dark	130	7	10–20		80	Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	modified Lin and Staba medium, 0.2 mg/L 2,4-D, pH 5.7	27	dark	130	7	10		80	Erlenmeyer flask (300-mL)
2017-10-01		Living cells	Available	modified Lin and Staba medium, 0.2 mg/L 2,4-D, pH 5.7	27	dark	130	7	10		80	Erlenmeyer flask (300-mL)
2019-02-07		Living cells	Available	modified Lin and Staba medium, 0.2 mg/L 2,4-D, pH 5.7	27	dark	130	7	10		80	Erlenmeyer flask (300-mL)
2020-02-05		Living cells	Available	modified Lin and Staba medium, 0.5 µM 2,4-D, pH 5.7	27	dark	130	7	10		80	Erlenmeyer flask (300-mL)
2022-06-16		Living cells	Available	modified Lin and Staba medium, 0.2 mg/L 2,4-D, pH 5.7	27	dark	130	7	10		80	Erlenmeyer flask (300-mL)
2023-11-01	Present	Living cells	Available	modified Lin and Staba medium, 0.2 mg/L 2,4-D, pH 5.7	27	dark	130	7	10		80	Erlenmeyer flask (300-mL)