Maintenance history: rpc00030

rpc00030: Zinnia elegans ZE3 callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	Murashige and Skoog medium, 10 µM NAA, 1.2% (w/v) agar, pH 6.2	27	dark		28			100	Erlenmeyer flask (300-mL)
2003-05-14	Release	Living cells	Available	Murashige and Skoog medium, 10 µM NAA, 1.2% (w/v) agar, pH 6.2	27	dark		28				Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	Murashige and Skoog medium, 10 µM NAA, 1.2% (w/v) agar, pH 6.2	27	dark		28				Erlenmeyer flask (200-mL)
2017-10-01		Living cells	Available	Murashige and Skoog medium, 10 µM NAA, 1.2% (w/v) agar, pH 6.2	27	dark		28		3 callus (diameter 5 mm)	80	Erlenmeyer flask (200-mL)
2019-02-07		Living cells	Available	Murashige and Skoog medium, 10 µM NAA, 1.2% (w/v) agar, pH 6.2	27	dark		28		3 callus (diameter 5 mm)	80	Erlenmeyer flask (200-mL)
2023-11-01	Present	Living cells	Available	Murashige and Skoog medium, 10 µM NAA, 1.2% (w/v) agar, pH 6.2	27	dark		28		3 callus (diameter 5 mm)	80	Erlenmeyer flask (200-mL)