Maintenance history: rpc00111
Date |
Description |
Cell status |
Previous availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
2012-05-18 |
Depositor |
Living cells |
Available |
modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8 |
26 |
dark |
130 |
7 |
10 |
settled cells |
100 |
Erlenmeyer flask (300-mL) |
2016-02-26 |
Induction |
Living cells |
Available |
modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, 0.4% (w/v) gellan gum, pH 5.8 |
27 |
dark |
14–21 |
9 callus (diameter 3 mm) |
30 |
Petri dish (9-cm) |
||
2024-03-13 |
Present |
Living cells |
Available |
modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, 0.4% (w/v) gellan gum, pH 5.8 |
27 |
dark |
14–21 |
9 callus (diameter 3 mm) |
30 |
Petri dish (9-cm) |