Maintenance history: rpc00020

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2003-05-14 RIKEN Cell Bank   AA medium, 1 mg/L 2,4-D, 0.2 mg/L kinetin, pH 5.8 27 dark 100 14 10   90 flask (500-mL)
2003-05-14     AA medium, 1 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 5.8 27 dark   28       flask (100-mL)
2014-11-07     AA medium, 1 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 5.8 27 dark   28     40 flask (100-mL)
2017-10-01     AA medium, 1 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 5.8 27 dark   28   2 callus (diameter 10 mm) 40 flask (100-mL)
2019-02-07 Present   AA medium, 1 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 5.8 27 dark   28   2 callus (diameter 10 mm) 40 flask (100-mL)