Maintenance history: rpc00087

rpc00087: Bruguiera sexangula BsLs cell suspension culture

Date	Description	Past availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2018-02-27	Depositor		Amino Acid medium, 0.02 µM 2,4-D, 2 µM 4-CPPU, pH 6.2	26	dark	128	21	2		20	culture bottle
2022-03-22	Release		Amino Acid medium, 0.02 µM 2,4-D, 2 µM 4-CPPU, pH 6.2	27	dark	130	21	2		20	Erlenmeyer flask (100-mL)
2023-11-01	Present		Amino Acid medium, 0.02 µM 2,4-D, 2 µM 4-CPPU, pH 6.2	27	dark	130	21	2		20	Erlenmeyer flask (100-mL)