Maintenance history: rpc00064
Date |
Description |
Past availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|
2015-02-10 |
Depositor |
Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.8 |
25 |
12 h light (3000-5000 lux)/12 h dark |
21–28 |
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2021-03-15 |
Release |
Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.8 |
25 |
12 h light (PPFD 37.5 µmol m-2 s-1)/12 h dark |
28 |
4-5 callus (diameter 5-6 mm) |
40 |
Erlenmeyer flask (100-mL) |
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2023-11-01 |
Present |
Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.8 |
25 |
12 h light (PPFD 37.5 µmol m-2 s-1)/12 h dark |
28 |
4-5 callus (diameter 5-6 mm) |
40 |
Erlenmeyer flask (100-mL) |