Maintenance history: rpc00064

Date

Description

Past availability

Medium

Temperature (°C)

Light condition

Rotation (rpm)

Subculture (days)

Transfer volume (mL)

Transfer method

Medium volume (mL)

Container

2015-02-10

Depositor

Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.8

25

12 h light (3000-5000 lux)/12 h dark

21–28

2021-03-15

Release

Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.8

25

12 h light (PPFD 37.5 µmol m-2 s-1)/12 h dark

28

4-5 callus (diameter 5-6 mm)

40

Erlenmeyer flask (100-mL)

2023-11-01

Present

Murashige and Skoog medium, 1 mg/L 2,4-D, 0.1 mg/L kinetin, 0.9% (w/v) agar, pH 5.8

25

12 h light (PPFD 37.5 µmol m-2 s-1)/12 h dark

28

4-5 callus (diameter 5-6 mm)

40

Erlenmeyer flask (100-mL)