Maintenance history: rpc00003

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2003-05-14 RIKEN Cell Bank   modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, pH 6.1 27 dark 120 7 10   72 flask (300-mL)
2003-05-14     modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 27 dark   28       flask (300-mL)
2014-11-07     modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 27 dark   56     100 flask (300-mL)
2017-10-01     modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 27 dark   56   2–3 callus (diameter 8 mm) 100 flask (300-mL)
2019-02-07 Present   modified Linsmaier and Skoog medium, 0.05 mg/L 2,4-D, 0.2 mg/L kinetin, 1.2% (w/v) agar, pH 6.1 27 dark   56   2–3 callus (diameter 8 mm) 100 flask (300-mL)