Maintenance history: rpc00044

rpc00044: Nicotiana glauca G89 callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2005-12-08	Depositor	Living cells	Available
2005-12-08	Datasheet	Living cells	Available	Murashige and Skoog medium, 1 mg/L IAA, 0.9% (w/v) agar, pH 5.7	27	dark		28			40	Erlenmeyer flask (100-mL)
2013-02-28	Release	Living cells	Available	Murashige and Skoog medium, 1 mg/L IAA, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (100-mL)
2014-11-07		Living cells	Available	Murashige and Skoog medium, 1 mg/L IAA, 0.9% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (200-mL)
2017-10-01		Living cells	Available	Murashige and Skoog medium, 1 mg/L IAA, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)
2019-02-07		Living cells	Available	Murashige and Skoog medium, 1 mg/L IAA, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)
2023-11-01	Present	Living cells	Available	Murashige and Skoog medium, 1 mg/L IAA, 0.9% (w/v) agar, pH 5.7	27	dark		28		3 callus (diameter 5 mm)	40	Erlenmeyer flask (100-mL)