Maintenance history: rpc00058

Date

Description

Past availability

Medium

Temperature (°C)

Light condition

Rotation (rpm)

Subculture (days)

Transfer volume (mL)

Transfer method

Medium volume (mL)

Container

2014-11-12

Depositor

Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8

25

dark

30–60

2018-03-30

Release

Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8

27

dark

28

3-4 callus (diameter 3-5 mm)

40

Erlenmeyer flask (100-mL)

2019-02-07

Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8

27

dark

28

3-4 callus (diameter 3-5 mm)

40

Erlenmeyer flask (100-mL)

2023-11-01

Present

Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8

27

dark

21–28

3-4 callus (diameter 3-5 mm)

40

Erlenmeyer flask (100-mL)