Maintenance history: rpc00058

rpc00058: Glehnia littoralis GlV callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2014-11-12	Depositor	Living cells	Available	Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8	25	dark		30–60
2018-03-30	Release	Living cells	Available	Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8	27	dark		28		3-4 callus (diameter 3-5 mm)	40	Erlenmeyer flask (100-mL)
2019-02-07		Living cells	Available	Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8	27	dark		28		3-4 callus (diameter 3-5 mm)	40	Erlenmeyer flask (100-mL)
2023-11-01	Present	Living cells	Available	Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8	27	dark		21–28		3-4 callus (diameter 3-5 mm)	40	Erlenmeyer flask (100-mL)