Maintenance history: rpc00058
Date |
Description |
Past availability |
Medium |
Temperature (°C) |
Light condition |
Rotation (rpm) |
Subculture (days) |
Transfer volume (mL) |
Transfer method |
Medium volume (mL) |
Container |
|---|---|---|---|---|---|---|---|---|---|---|---|
2014-11-12 |
Depositor |
Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 |
25 |
dark |
30–60 |
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2018-03-30 |
Release |
Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 |
27 |
dark |
28 |
3-4 callus (diameter 3-5 mm) |
40 |
Erlenmeyer flask (100-mL) |
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2019-02-07 |
Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 |
27 |
dark |
28 |
3-4 callus (diameter 3-5 mm) |
40 |
Erlenmeyer flask (100-mL) |
||||
2023-11-01 |
Present |
Gamborgʼs B5 medium, 1 mg/L NAA, 0.01 mg/L kinetin, 0.8% (w/v) agar, pH 5.8 |
27 |
dark |
21–28 |
3-4 callus (diameter 3-5 mm) |
40 |
Erlenmeyer flask (100-mL) |