Maintenance history: rpc00025

rpc00025: Sesamum indicum PSB callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 0.2 mg/L BAP, 1.2% (w/v) agar, pH 5.7	27	dark		28			80	Erlenmeyer flask (300-mL)
2003-05-14	Release	Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 0.2 mg/L BAP, 1.2% (w/v) agar, pH 5.7	27	dark		28				Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 0.2 mg/L BAP, 1.2% (w/v) agar, pH 5.7	27	dark		28–42			80	Erlenmeyer flask (200-mL)
2017-10-01		Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 0.2 mg/L BAP, 1.2% (w/v) agar, pH 5.7	27	dark		28–42		3 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)
2019-02-07		Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 0.2 mg/L BAP, 1.2% (w/v) agar, pH 5.7	27	dark		28–42		3 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)
2023-11-01	Present	Living cells	Available	Linsmaier and Skoog medium, 1 µM 2,4-D, 0.2 mg/L BAP, 1.2% (w/v) agar, pH 5.7	27	dark		28–42		3 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)