Maintenance history: rpc00013

Date

Description

Past availability

Medium

Temperature (°C)

Light condition

Rotation (rpm)

Subculture (days)

Transfer volume (mL)

Transfer method

Medium volume (mL)

Container

2002-04-01

RIKEN Cell Bank

Linsmaier and Skoog medium, 1 µM 2,4-D, 1 µM kinetin, pH 5.7

27

dark

120

7

10

10

Erlenmeyer flask (300-mL)

2003-05-14

Release

Linsmaier and Skoog medium, 1 µM 2,4-D, 1 µM kinetin, 1.2% (w/v) agar, pH 5.7

27

dark

28

Erlenmeyer flask (200-mL)

2014-11-07

Linsmaier and Skoog medium, 1 µM 2,4-D, 1 µM kinetin, 1.2% (w/v) agar, pH 5.7

27

dark

28

80

Erlenmeyer flask (200-mL)

2017-10-01

Linsmaier and Skoog medium, 1 µM 2,4-D, 1 µM kinetin, 1.2% (w/v) agar, pH 5.7

27

dark

28

3 callus (diameter 8 mm)

80

Erlenmeyer flask (200-mL)

2019-02-07

Linsmaier and Skoog medium, 1 µM 2,4-D, 1 µM kinetin, 1.2% (w/v) agar, pH 5.7

27

dark

28–42

3 callus (diameter 8 mm)

80

Erlenmeyer flask (200-mL)

2023-11-01

Present

Linsmaier and Skoog medium, 1 µM 2,4-D, 1 µM kinetin, 1.2% (w/v) agar, pH 5.7

27

dark

28–42

3 callus (diameter 8 mm)

80

Erlenmeyer flask (200-mL)