Maintenance history: rpc00001

rpc00001: Nicotiana tabacum BY-2 cell suspension culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2–1.4		95	Erlenmeyer flask (300-mL)
2003-05-14	Release	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2–1.4		95	Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.2		95	Erlenmeyer flask (300-mL)
2017-10-01		Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.2		95	Erlenmeyer flask (300-mL)
2019-02-07		Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.2		95	Erlenmeyer flask (300-mL)
2023-11-01	Present	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.2		95	Erlenmeyer flask (300-mL)
2024-01-26	Present	Cryopreserved cells	Backup	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.2		95	Erlenmeyer flask (300-mL)