Maintenance history: rpc00054

rpc00054: Coptis japonica Cj callus culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2009-06-10	Depositor	Living cells	Available	Linsmaier and Skoog medium, 100 µM NAA, 1 µM BAP, 1% (w/v) agar, pH 6.0	25	dark		30–31
2009-06-10	Datasheet	Living cells	Available	Linsmaier and Skoog medium, 100 µM NAA, 1 µM BAP, 1% (w/v) agar, pH 6.0	25	dark		28			40	Erlenmeyer flask (100-mL)
2015-11-13	Release	Living cells	Available	Linsmaier and Skoog medium, 100 µM NAA, 1 µM BAP, 1% (w/v) agar, pH 6.0	25	dark		28				Erlenmeyer flask (100-mL)
2017-10-01		Living cells	Available	Linsmaier and Skoog medium, 100 µM NAA, 1 µM BAP, 1% (w/v) agar, pH 6.0	27	dark		56		4 callus (diameter 7 mm)	40	Erlenmeyer flask (100-mL)
2019-02-07		Living cells	Available	Linsmaier and Skoog medium, 100 µM NAA, 1 µM BAP, 1% (w/v) agar, pH 6.0	27	dark		56		4 callus (diameter 7 mm)	40	Erlenmeyer flask (100-mL)
2023-11-01	Present	Living cells	Available	Linsmaier and Skoog medium, 100 µM NAA, 1 µM BAP, 1% (w/v) agar, pH 6.0	27	dark		56		4 callus (diameter 7 mm)	40	Erlenmeyer flask (100-mL)