Maintenance history: rpc00039

rpc00039: Nicotiana tabacum GV7 transgenic cell suspension culture

Date	Description	Cell status	Previous availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2008-06-13	Depositor	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1–1.5		95
2008-06-13	Datasheet	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.5		95	Erlenmeyer flask (300-mL)
2011-09-16	Release	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.5		95	Erlenmeyer flask (300-mL)
2014-11-07		Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2		95	Erlenmeyer flask (300-mL)
2017-10-01		Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2		95	Erlenmeyer flask (300-mL)
2019-02-07		Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2		95	Erlenmeyer flask (300-mL)
2023-11-01	Present	Living cells	Available	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2		95	Erlenmeyer flask (300-mL)
2024-11-15	Present	Cryopreserved cells	Backup	modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-D, pH 5.8	27	dark	130	7	1.2		95	Erlenmeyer flask (300-mL)