Maintenance history: rpc00007

Date Description Past avaliability Medium Temperature (°C) Light condition Rotation (rpm) Subculture (days) Transfer volume (mL) Transfer method Medium volume (mL) Container
2003-05-14 RIKEN Cell Bank   Murashige and Skoog medium, 5 µM 2,4-D, pH 5.8 27 dark 120 7 10   72 flask (300-mL)
2003-05-14     Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8 27 dark   28       flask (300-mL)
2014-11-07     Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8 27 dark   28     100 flask (300-mL)
2017-10-01     Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8 27 dark   28   3 callus (diameter 8 mm) 100 flask (300-mL)
2019-02-07 Present   Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8 27 dark   28   3 callus (diameter 8 mm) 100 flask (300-mL)