Maintenance history: rpc00023

rpc00023: Asparagus pastorianus A.pas callus culture

Date	Description	Past availability	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank		Linsmaier and Skoog medium, 10 µM 2,4-D, 1.2% (w/v) agar, pH 5.6	27	dark		28			80	Erlenmeyer flask (200-mL)
2003-05-14	Release		Linsmaier and Skoog medium, 10 µM 2,4-D, 1.2% (w/v) agar, pH 5.6	27	dark		28				Erlenmeyer flask (200-mL)
2014-11-07			Linsmaier and Skoog medium, 10 µM 2,4-D, 1.2% (w/v) agar, pH 5.6	27	dark		28			80	Erlenmeyer flask (200-mL)
2017-10-01			Linsmaier and Skoog medium, 10 µM 2,4-D, 1.2% (w/v) agar, pH 5.6	27	dark		28		3 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)
2019-02-07			Linsmaier and Skoog medium, 10 µM 2,4-D, 1.2% (w/v) agar, pH 5.6	27	dark		28		3 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)
2023-11-01	Present		Linsmaier and Skoog medium, 10 µM 2,4-D, 1.2% (w/v) agar, pH 5.6	27	dark		28–42		3 callus (diameter 10 mm)	80	Erlenmeyer flask (200-mL)