Maintenance history: rpc00005

rpc00005: Phytolacca americana PAR callus culture

Date	Description	Medium	Temperature (°C)	Light condition	Rotation (rpm)	Subculture (days)	Transfer volume (mL)	Transfer method	Medium volume (mL)	Container
2002-04-01	RIKEN Cell Bank	Murashige and Skoog medium, 5 µM 2,4-D, pH 5.8	27	dark	120	7	10		72	Erlenmeyer flask (300-mL)
2003-05-14	Release	Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8	27	dark		28				Erlenmeyer flask (300-mL)
2014-11-07		Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8	27	dark		28			100	Erlenmeyer flask (300-mL)
2017-10-01		Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8	27	dark		28		3 callus (diameter 10 mm)	100	Erlenmeyer flask (300-mL)
2019-02-07		Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8	27	dark		28		3 callus (diameter 10 mm)	100	Erlenmeyer flask (300-mL)
2023-11-01	Present	Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8	27	dark		28		3 callus (diameter 10 mm)	100	Erlenmeyer flask (300-mL)