Maintenance history: rpc00005

Date

Description

Past availability

Medium

Temperature (°C)

Light condition

Rotation (rpm)

Subculture (days)

Transfer volume (mL)

Transfer method

Medium volume (mL)

Container

2002-04-01

RIKEN Cell Bank

Murashige and Skoog medium, 5 µM 2,4-D, pH 5.8

27

dark

120

7

10

72

Erlenmeyer flask (300-mL)

2003-05-14

Release

Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8

27

dark

28

Erlenmeyer flask (300-mL)

2014-11-07

Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8

27

dark

28

100

Erlenmeyer flask (300-mL)

2017-10-01

Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8

27

dark

28

3 callus (diameter 10 mm)

100

Erlenmeyer flask (300-mL)

2019-02-07

Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8

27

dark

28

3 callus (diameter 10 mm)

100

Erlenmeyer flask (300-mL)

2023-11-01

Present

Murashige and Skoog medium, 1 mg/L 2,4-D, 1.2% (w/v) agar, pH 5.8

27

dark

28

3 callus (diameter 10 mm)

100

Erlenmeyer flask (300-mL)