medium no. 1: modified Linsmaier and Skoog medium, 0.2 mg/L 2,4-dichlorophenoxyacetic acid, pH 5.8
Plant cell lines
rpc00039: Nicotiana tabacum GV7 transgenic cell suspension culture
rpc00040: Nicotiana tabacum GF11 transgenic cell suspension culture
rpc00041: Nicotiana tabacum GT16 transgenic cell suspension culture
rpc00056: Arabidopsis thaliana At tom cell suspension culture
rpc00062: Nicotiana tabacum BY-TIPG transgenic cell suspension culture
rpc00091: Nicotiana tabacum TBY2-31/ST transgenic cell suspension culture
rpc00092: Nicotiana tabacum TBY2-31/ST(E) transgenic cell suspension culture
rpc00093: Nicotiana tabacum TBY2-41/ST transgenic cell suspension culture
rpc00095: Nicotiana tabacum TBY2-R31 transgenic cell suspension culture
rpc00097: Nicotiana tabacum TBY2-31/41 transgenic cell suspension culture
rpc00102: Ceratopteris richardii Cr-AH cell suspension culture
rpc00104: Arabidopsis thaliana MM2d-LS cell suspension culture
Chemicals and stock solutions
(All stock solutions are stored at 4°C)
MS salt mix
Murashige and Skoog Plant Salt Mixture, FUJIFILM Wako Pure Chemical Corporation (#392-00591)
Sucrose
BY2_P
Chemical
Concentration (mg/mL)
KH2PO4
80
LS_VT_modified
Chemical
Concentration (mg/mL)
Thiamine·HCl
0.4
myo-Inositol
40
2,4-D (0.2 mg/mL)
Chemical
Concentration (mg/mL)
2,4-D sodium monohydrate
0.236
(2,4-Dichlorophenoxy)acetic acid sodium salt monohydrate, Sigma-Aldrich (D6679)
KOH (1 N)
Medium preparation
Dissolve the following chemicals in approximately 800 mL of distilled water.
Chemical
Amount
MS salt mix
1 bag (1 L)
Sucrose
30 g
Add following stock solutions, and fill up to approximately 950 mL with distilled water.
Stock solution
Volume (mL)
BY2_P
2.5
LS_VT_modified
2.5
2,4-D (0.2 mg/mL)
1
Adjust the pH of the solution to 5.8 with KOH (1 N), and fill up to 1 L with distilled water.
Autoclave the medium at 121°C for 20 min.
Formulation
Chemical
Concentration (mg/L)
KNO3
1900
NH4NO3
1650
CaCl2·2H2O
440
MgSO4·7H2O
370
KH2PO4
370
H3BO3
6.2
MnSO4·4H2O
22.3
ZnSO4·7H2O
8.6
KI
0.83
Na2MoO4·2H2O
0.25
CuSO4·5H2O
0.025
CoCl2·6H2O
0.025
FeSO4·7H2O
27.8
Na2-EDTA
37.3
Thiamine·HCl
1
myo-Inositol
100
Sucrose
30000
2,4-D sodium monohydrate
0.236