medium no. 24: Linsmaier and Skoog medium, 1 µM 2,4-dichlorophenoxyacetic acid, 0.2 mg/L 6-benzylaminopurine, 1.2% (w/v) agar, pH 5.6

Chemicals and stock solutions

(All stock solutions are stored at 4°C)
  • MS salt mix

    Murashige and Skoog Plant Salt Mixture, Wako Pure Chemical Industries (#392-00591)

  • Glucose

  • LS_VT

    Chemical Concentration (mg/mL)
    Thiamine·HCl 0.16
    myo-Inositol 40
  • 2,4-D (1 mM)

    Chemical Concentration (mg/mL)
    2,4-D sodium monohydrate 0.261

    (2,4-Dichlorophenoxy)acetic acid sodium salt monohydrate, Sigma-Aldrich (D6679)

  • BAP (1 mg/mL)

    Chemical Concentration (mg/mL)
    6-Benzylaminopurine 1

    Dissolve 6-benzylaminopurine in small volume of KOH (1 N), and fill up with distilled water

  • Agar, powder

  • KOH (1 N)

Medium preparation

  1. Dissolve the following chemicals in approximately 800 mL of distilled water.

    Chemical Amount
    MS salt mix 1 bag (1 L)
    Glucose 30 g
  2. Add following stock solutions, and fill up to approximately 950 mL with distilled water.

    Stock solution Volume (mL)
    LS_VT 2.5
    2,4-D (1 mM) 1
    BAP (1 mg/mL) 0.2
  3. Adjust the pH of the solution to 5.6 with KOH (1 N), and fill up to 1 L with distilled water.

  4. Add 12 g of agar to the medium.

  5. Autoclave the medium at 121°C for 20 min.

Formulation

Chemical Concentration (mg/L)
KNO3 1900
NH4NO3 1650
CaCl2·2H2O 440
MgSO4·7H2O 370
KH2PO4 170
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
KI 0.83
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA 37.3
Thiamine·HCl 0.4
myo-Inositol 100
Glucose 30000
2,4-D sodium monohydrate 0.261
6-Benzylaminopurine 0.2
Agar 12000