medium no. 46: Murashige and Skoog medium, 10 µM 1-naphthaleneacetic acid, 0.9% (w/v) agar, pH 6.2

Plant cell lines

Chemicals and stock solutions

(All stock solutions are stored at 4°C)
  • MS salt mix

    Murashige and Skoog Plant Salt Mixture, Wako Pure Chemical Industries (#392-00591)

  • Sucrose

  • MS_VT

    Chemical Concentration (mg/mL)
    Nicotinic acid 0.5
    Pyridoxine·HCl 0.5
    Thiamine·HCl 0.1
    Glycine 2
  • MS_inositol

    Chemical Concentration (mg/mL)
    myo-Inositol 40
  • NAA (1 mM)

    Chemical Concentration (mg/mL)
    NAA·K 0.224

    Potassium 1-naphthylacetate, Wako Pure Chemical Industries (#161-04021)

  • Agar, powder

  • KOH (1 N)

Medium preparation

  1. Dissolve the following chemicals in approximately 800 mL of distilled water.

    Chemical Amount
    MS salt mix 1 bag (1 L)
    Sucrose 30 g
  2. Add following stock solutions, and fill up to approximately 950 mL with distilled water.

    Stock solution Volume (mL)
    MS_VT 1
    MS_inositol 2.5
    NAA (1 mM) 10
  3. Adjust the pH of the solution to 6.2 with KOH (1 N), and fill up to 1 L with distilled water.

  4. Add 9 g of agar to the medium.

  5. Autoclave the medium at 121°C for 20 min.

Formulation

Chemical Concentration (mg/L)
KNO3 1900
NH4NO3 1650
CaCl2·2H2O 440
MgSO4·7H2O 370
KH2PO4 170
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
KI 0.83
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA 37.3
Nicotinic acid 0.5
Pyridoxine·HCl 0.5
Thiamine·HCl 0.1
Glycine 2
myo-Inositol 100
Sucrose 30000
NAA·K 2.24
Agar 9000