medium no. 12: modified Murashige and Skoog medium, 0.5 mg/L 1-naphthaleneacetic acid, 1 mg/L 6-benzylaminopurine, pH 6.5

Chemicals and stock solutions

(All stock solutions are stored at 4°C)
  • MS salt mix

    Murashige and Skoog Plant Salt Mixture, Wako Pure Chemical Industries (#392-00591)

  • Sucrose

  • MS_VT

    Chemical Concentration (mg/mL)
    Nicotinic acid 0.5
    Pyridoxine·HCl 0.5
    Thiamine·HCl 0.1
    Glycine 2
  • MS_inositol

    Chemical Concentration (mg/mL)
    myo-Inositol 40
  • NAA (1 mg/mL)

    Chemical Concentration (mg/mL)
    NAA·K 1.2

    Potassium 1-naphthylacetate, Wako Pure Chemical Industries (# 161-04021)

  • BAP (1 mg/mL)

    Chemical Concentration (mg/mL)
    6-Benzylaminopurine 1

    Dissolve 6-benzylaminopurine in small volume of KOH (1 N), and fill up with distilled water

  • NaOH (1 N)

Medium preparation

  1. Dissolve the following chemicals in approximately 800 mL of distilled water.

    Chemical Amount
    MS salt mix 1 bag (1 L)
    Sucrose 20 g
  2. Add following stock solutions, and fill up to approximately 950 mL with distilled water.

    Stock solution Volume (mL)
    MS_VT 1
    MS_inositol 2.5
    NAA (1 mg/mL) 0.5
    BAP (1 mg/mL) 1
  3. Adjust the pH of the solution to 6.5 with NaOH (1 N), and fill up to 1 L with distilled water.

  4. Autoclave the medium at 121°C for 20 min.

Formulation

Chemical Concentration (mg/L)
KNO3 1900
NH4NO3 1650
CaCl2·2H2O 440
MgSO4·7H2O 370
KH2PO4 170
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
KI 0.83
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA 37.3
Nicotinic acid 0.5
Pyridoxine·HCl 0.5
Thiamine·HCl 0.1
Glycine 2
myo-Inositol 100
Sucrose 20000
NAA·K 0.6
6-Benzylaminopurine 1