medium no. 43: modified Murashige and Skoog medium, 1 mg/L 2,4-dichlorophenoxyacetic acid, 0.8% (w/v) agar, pH 5.8

Chemicals and stock solutions

(All stock solutions are stored at 4°C)
  • MS salt mix

    Murashige and Skoog Plant Salt Mixture, Wako Pure Chemical Industries (#392-00591)

  • Sucrose

  • KH2PO4 (100 mg/mL)

    Chemical Concentration (mg/mL)
    KH2PO4 100
  • B5_VT

    Chemical Concentration (mg/mL)
    Nicotinic acid 0.4
    Pyridoxine·HCl 0.4
    Thiamine·HCl 4
    myo-Inositol 40
  • 2,4-D (0.2 mg/mL)

    Chemical Concentration (mg/mL)
    2,4-D sodium monohydrate 0.236

    (2,4-Dichlorophenoxy)acetic acid sodium salt monohydrate, Sigma-Aldrich (D6679)

  • Agar, powder

  • KOH (1 N)

Medium preparation

  1. Dissolve the following chemicals in approximately 800 mL of distilled water.

    Chemical Amount
    MS salt mix 1 bag (1 L)
    Sucrose 20 g
  2. Add following stock solutions, and fill up to approximately 950 mL with distilled water.

    Stock solution Volume (mL)
    KH2PO4 (100 mg/mL) 3.4
    B5_VT 5
    2,4-D (0.2 mg/mL) 5
  3. Adjust the pH of the solution to 5.8 with KOH (1 N), and fill up to 1 L with distilled water.

  4. Add 8 g of agar to the medium.

  5. Autoclave the medium at 121°C for 20 min.

Formulation

Chemical Concentration (mg/L)
KNO3 1900
NH4NO3 1650
CaCl2·2H2O 440
MgSO4·7H2O 370
KH2PO4 510
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
KI 0.83
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA 37.3
Nicotinic acid 2
Pyridoxine·HCl 2
Thiamine·HCl 20
myo-Inositol 200
Sucrose 20000
2,4-D sodium monohydrate 1.18
Agar 8000