medium no. 32: Linsmaier and Skoog medium, 1 µM indole-3-acetic acid, 10 µM kinetin, 1% (w/v) agar, pH 6.3

Chemicals and stock solutions

(All stock solutions are stored at 4°C)
  • MS salt mix

    Murashige and Skoog Plant Salt Mixture, Wako Pure Chemical Industries (#392-00591)

  • Sucrose

  • LS_VT

    Chemical Concentration (mg/mL)
    Thiamine·HCl 0.16
    myo-Inositol 40
  • IAA (1 mM)

    Chemical Concentration (mg/mL)
    IAA·K 0.213

    Potassium 3-indoleacetate, Wako Pure Chemical Industries (#160-07531)

  • Kinetin (1 mM)

    Chemical Concentration (mg/mL)
    Kinetin 0.215

    Dissolve kinetin in small volume of KOH (1 N), and fill up with distilled water

  • Agar, powder

  • NaOH (1 N)

Medium preparation

  1. Dissolve the following chemicals in approximately 800 mL of distilled water.

    Chemical Amount
    MS salt mix 1 bag (1 L)
    Sucrose 30 g
  2. Add following stock solutions, and fill up to approximately 950 mL with distilled water.

    Stock solution Volume (mL)
    LS_VT 2.5
    IAA (1 mM) 1
    Kinetin (1 mM) 10
  3. Adjust the pH of the solution to 6.3 with NaOH (1 N), and fill up to 1 L with distilled water.

  4. Add 10 g of agar to the medium.

  5. Autoclave the medium at 121°C for 20 min.

Formulation

Chemical Concentration (mg/L)
KNO3 1900
NH4NO3 1650
CaCl2·2H2O 440
MgSO4·7H2O 370
KH2PO4 170
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
KI 0.83
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA 37.3
Thiamine·HCl 0.4
myo-Inositol 100
Sucrose 30000
IAA·K 0.213
Kinetin 2.15
Agar 10000