=========================================================================================================== medium no. 32: Linsmaier and Skoog medium, 1 µM indole-3-acetic acid, 10 µM kinetin, 1% (w/v) agar, pH 6.3 =========================================================================================================== Plant cell lines ================= * :doc:`/cell_lines/rpc00037` Chemicals and stock solutions ============================== (All stock solutions are stored at 4°C) * MS salt mix Murashige and Skoog Plant Salt Mixture, FUJIFILM Wako Pure Chemical Corporation (#392-00591) * Sucrose * LS_VT .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "Thiamine·HCl", "0.16" "*myo*-Inositol", "40" * IAA (1 mM) .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "IAA·K", "0.213" Potassium 3-indoleacetate, FUJIFILM Wako Pure Chemical Corporation (#160-07531) * Kinetin (1 mM) .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "Kinetin", "0.215" Dissolve kinetin in small volume of KOH (1 N), and fill up with distilled water * Agar, powder * NaOH (1 N) Medium preparation =================== #. Dissolve the following chemicals in approximately 800 mL of distilled water. .. csv-table:: :header: "Chemical", "Amount" :widths: auto "MS salt mix", "1 bag (1 L)" "Sucrose", "30 g" #. Add following stock solutions, and fill up to approximately 950 mL with distilled water. .. csv-table:: :header: "Stock solution", "Volume (mL)" :widths: auto "LS_VT", "2.5" "IAA (1 mM)", "1" "Kinetin (1 mM)", "10" #. Adjust the pH of the solution to 6.3 with NaOH (1 N), and fill up to 1 L with distilled water. #. Add 10 g of agar to the medium. #. Autoclave the medium at 121°C for 20 min. Formulation ============ .. csv-table:: :header: "Chemical", "Concentration (mg/L)" :widths: auto "KNO\ :sub:`3`", "1900" "NH\ :sub:`4`\ NO\ :sub:`3`", "1650" "CaCl\ :sub:`2`\ ·2H\ :sub:`2`\ O", "440" "MgSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "370" "KH\ :sub:`2`\ PO\ :sub:`4`", "170" "H\ :sub:`3`\ BO\ :sub:`3`", "6.2" "MnSO\ :sub:`4`\ ·4H\ :sub:`2`\ O", "22.3" "ZnSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "8.6" "KI", "0.83" "Na\ :sub:`2`\ MoO\ :sub:`4`\ ·2H\ :sub:`2`\ O", "0.25" "CuSO\ :sub:`4`\ ·5H\ :sub:`2`\ O", "0.025" "CoCl\ :sub:`2`\ ·6H\ :sub:`2`\ O", "0.025" "FeSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "27.8" "Na\ :sub:`2`\ -EDTA", "37.3" "Thiamine·HCl", "0.4" "*myo*-Inositol", "100" "Sucrose", "30000" "IAA·K", "0.213" "Kinetin", "2.15" "Agar", "10000"