========================================================================================================================== medium no. 12: modified Murashige and Skoog medium, 0.5 mg/L 1-naphthaleneacetic acid, 1 mg/L 6-benzylaminopurine, pH 6.5 ========================================================================================================================== Plant cell lines ================= * :doc:`/cell_lines/rpc00016` Chemicals and stock solutions ============================== (All stock solutions are stored at 4°C) * MS salt mix Murashige and Skoog Plant Salt Mixture, FUJIFILM Wako Pure Chemical Corporation (#392-00591) * Sucrose * MS_VT .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "Nicotinic acid", "0.5" "Pyridoxine·HCl", "0.5" "Thiamine·HCl", "0.1" "Glycine", "2" * MS_inositol .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "*myo*-Inositol", "40" * NAA (1 mg/mL) .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "NAA·K", "1.2" Potassium 1-naphthylacetate, FUJIFILM Wako Pure Chemical Corporation (#161-04021) * BAP (1 mg/mL) .. csv-table:: :header: "Chemical", "Concentration (mg/mL)" :widths: auto "6-Benzylaminopurine", "1" Dissolve 6-benzylaminopurine in small volume of KOH (1 N), and fill up with distilled water * NaOH (1 N) Medium preparation =================== #. Dissolve the following chemicals in approximately 800 mL of distilled water. .. csv-table:: :header: "Chemical", "Amount" :widths: auto "MS salt mix", "1 bag (1 L)" "Sucrose", "20 g" #. Add following stock solutions, and fill up to approximately 950 mL with distilled water. .. csv-table:: :header: "Stock solution", "Volume (mL)" :widths: auto "MS_VT", "1" "MS_inositol", "2.5" "NAA (1 mg/mL)", "0.5" "BAP (1 mg/mL)", "1" #. Adjust the pH of the solution to 6.5 with NaOH (1 N), and fill up to 1 L with distilled water. #. Autoclave the medium at 121°C for 20 min. Formulation ============ .. csv-table:: :header: "Chemical", "Concentration (mg/L)" :widths: auto "KNO\ :sub:`3`", "1900" "NH\ :sub:`4`\ NO\ :sub:`3`", "1650" "CaCl\ :sub:`2`\ ·2H\ :sub:`2`\ O", "440" "MgSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "370" "KH\ :sub:`2`\ PO\ :sub:`4`", "170" "H\ :sub:`3`\ BO\ :sub:`3`", "6.2" "MnSO\ :sub:`4`\ ·4H\ :sub:`2`\ O", "22.3" "ZnSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "8.6" "KI", "0.83" "Na\ :sub:`2`\ MoO\ :sub:`4`\ ·2H\ :sub:`2`\ O", "0.25" "CuSO\ :sub:`4`\ ·5H\ :sub:`2`\ O", "0.025" "CoCl\ :sub:`2`\ ·6H\ :sub:`2`\ O", "0.025" "FeSO\ :sub:`4`\ ·7H\ :sub:`2`\ O", "27.8" "Na\ :sub:`2`\ -EDTA", "37.3" "Nicotinic acid", "0.5" "Pyridoxine·HCl", "0.5" "Thiamine·HCl", "0.1" "Glycine", "2" "*myo*-Inositol", "100" "Sucrose", "20000" "NAA·K", "0.6" "6-Benzylaminopurine", "1"