Solanum lycopersicum (tomato) cell lines

Plant cell lines

• rpc00012: Solanum lycopersicum Sly-1 callus culture

• rpc00045: Solanum lycopersicum GCR26 cell suspension culture

• rpc00046: Solanum lycopersicum GCR237 cell suspension culture

Genotypes

Cell line	rbcL	matK	Tm-1
Sly-1	Solanum lycopersicum	Sly-1	—
GCR26	Solanum lycopersicum	GCR	+/+
GCR237	Solanum lycopersicum	GCR	Tm-1/Tm-1

Protocols

DNA barcoding — rbcL

DNA barcoding — rbcL

DNA barcoding — matK

1. PCR

   • Primer set 《Primer sequences》

     Target	Forward primer	Reverse primer	Product size
     matK	matK_F	matK_R	900 bp

   • PCR reaction mixture

     Component	10 µL reaction
     TaKaRa Ex Taq™ Premix	5 µL
     Forward primer (100 µM)	0.125 µL
     Reverse primer (100 µM)	0.125 µL
     Template DNA	1 µL
     Sterile distilled water	3.75 µL

   • PCR reaction

     Temperature	Time	Cycles
     94°C	1 min
     94°C	30 sec	35 cycles
     55°C	30 sec
     72°C	1 min
     72°C	10 min

2. Check the sequence of the PCR product.

   • NCBI BLAST search

   • Compare the DNA sequence with that of previous years.

DNA sequencing — Tm-1 gene

1. PCR

   • Primer set 《Primer sequences》

     Target	Forward primer	Reverse primer	PCR product size
     Tm-1	GCR_F2	GCRseq_R1	600 bp

   • PCR reaction mixture

     Component	10 µL reaction
     TaKaRa Ex Taq™ Premix	5 µL
     Forward primer (100 µM)	0.125 µL
     Reverse primer (100 µM)	0.125 µL
     Template DNA	1 µL
     Sterile distilled water	3.75 µL

   • PCR reaction

     Temperature	Time	Cycles
     94°C	1 min
     94°C	30 sec	35 cycles
     55°C	30 sec
     72°C	1 min
     72°C	7 min

2. Check the DNA sequence of the PCR product.