Primer sequences
DNA barcoding
Primer name
Direction
Sequence
13
Forward
ATGTCACCACAAACAGAGACTAAAGC
18
Reverse
GCAGCAGCTAGTTCCGGGCTCCA
2250
Forward
TTGGGAGTTCCTATCGTAATGCATG
2356
Reverse
CATTCATAAACTGCTCTACCATAGT
2384
Reverse
ACGTAAAGCTTTAGCTAGTACACGG
2385
Forward
AAACTTTCCAGGGACCACCTCATGG
atpF_f
Forward
ACTCGCACACACTCCCTTTCC
atpH_r
Reverse
GCTTTTATGGAAGCTTTAACAAT
Lj_atpF_seqR2
Reverse
CCCCCTTTTTGGGTTTTAGA
matK_F
Forward
ACCCAGTCCATCTGGAAATCTTGGTTC
matK_F1
Forward
TTACGATCTATTCATTCAATATTTCC
matK_R
Reverse
CGTACAGTACTTTTGTGTTTACGAG
matK_R1
Reverse
CAAATGGGTTTACTAATAGGATGCC
psbK_f
Forward
TTAGCCTTTGTTTGGCAAG
psbI_r
Reverse
AGAGTTTGAGAGTAAGCAT
rbcL-R
Reverse
TGTCTTCGCGGTGGACTTGATTTTAC
Transgene insertion
Primer name
Direction
Sequence
046D04_flankingseq_side
Reverse
AGAACATGCAGCCAGAGGTT
046D04_RBside
Forward
TCATTTGTGGCAGGAAAACA
2625
Forward
ATAATAACGCTGCGGACATCTACATTTT
35Spro_F2
Forward
CAGTGGTCCCAAAGATGGAC
35Spro_F3
Forward
ATTGATGTGATATCTCCACTGACGT
35Spro_F5
Forward
TTCGCAAGACCCTTCCTCTA
GF11_R
Reverse
CCGCTCAATGAGGAGA
GFP_494_F
Forward
ACTTCAAGATCCGCCACAAC
GFP_F
Forward
ACGTAAACGGCCACAAGTTC
GFP_R
Reverse
GTCCTCCTTGAAGTCGATGC
GFPS65T_F
Forward
CACATGAAGCAGCACGACTT
GT16_R
Reverse
CACCTTCCTCCATACC
GV7_R
Reverse
TCCTTGTGAAGTTGCA
mRFPstop_R
Reverse
TAAGCTTAGGCGCCGGTGGAGT
NOSter_R1
Reverse
GATCTAGTAACATAGATGACACC
NtTIP1_F
Forward
CCTCTATAGCGCATGCCTTC
pTiT37-F
Forward
gtagctcttgcccagcagac
pTiT37-R
Reverse
ctgcttaactctggccttgg
RER1B_R
Reverse
GCTGTTCGCAGAAGATTTCC
RER1B_R1
Reverse
CCCTCCATGCCTCATGTACT
SMT_R
Reverse
CTTAGGCCAAAACCCACAAA
SYP31stop_R
Reverse
GTCGACTTAAGCCACAAAGAAGA
SYP41stop_R
Reverse
CGTCGACTCACAAGAATATTTCC
DNA sequencing
Primer name
Direction
Sequence
145_F
Forward
AATGAGGACGATGACGATGAC
145_R
Reverse
TCCAGTACCCCTTCTCAACA
GCR_F2
Forward
CAGCYGGAAGGAGACAAATAG
GCRseq_R1
Reverse
GGCATGGAACACCAAAGTCT
gnom_At1g133980_F
Forward
TGAGTACACGCCTTTCTGGA
gnom_At1g133980_R
Reverse
CAAGAGCAGCATCCTTGTTG
PCRpsbALEFT
Forward
PCRpsbARIGHT
Reverse
Sp_NR_F6
Forward
TGATAACGTCACTCCTTCATACG
Sp_NR_R1
Reverse
ATTGCCATTCCATGTGAAAA
Spi8_F
Forward
CCTGTCGAGGGTGATTTTGT
Spi8_R
Reverse
CCAAAGCCAAAGAGATGTGC
tom1-2_F
Forward
TGTTGCAATTTCTGACCTTTTG
tom1-2_R
Reverse
GCAAAGAGCCTGCATTACCT
Tom3-1_F
Forward
AAGAGGTGATTCAAATCCAGAGA
Tom3-1_R
Reverse
TGAGCCAAATCAATCAATCAA
Fragment analysis
Primer name
Direction
Sequence
3483
Forward
gtgtcttAAAGAGGAAACCGTGTCTCG
3484
Reverse
cccccccccccccctgaGAGATAACCAAACACGCAACAA
3493
Forward
gtgtcttTCGTGCATGCTTTTCTTGAC
3494
Reverse
cccccccccccccctgaCCACCTAGTCTCACCCGTTT
3495
Forward
gtgtcttTCCCCTTTTGCTTCTTACCC
3496
Reverse
cccccccccccccctgaCAACAGATTCCTTGCTTGATGA
3497
Forward
gtgtcttGAGACGTACCAGGACATTGTTGT
3498
Reverse
cccccccccccccctgaGCATACTGAATGTCATGAAAGTGG
uni-FAM [1]
cccccccccccccctga