Transgene insertion
Plant cell lines
rpc00039: Nicotiana tabacum GV7 transgenic cell suspension culture
rpc00040: Nicotiana tabacum GF11 transgenic cell suspension culture
rpc00041: Nicotiana tabacum GT16 transgenic cell suspension culture
rpc00042: Nicotiana tabacum TBY2-AtRER1B transgenic callus culture
rpc00062: Nicotiana tabacum BY-TIPG transgenic cell suspension culture
rpc00069: Coptis japonica 156-S transgenic cell suspension culture
rpc00091: Nicotiana tabacum TBY2-31/ST transgenic cell suspension culture
rpc00093: Nicotiana tabacum TBY2-41/ST transgenic cell suspension culture
rpc00095: Nicotiana tabacum TBY2-R31 transgenic cell suspension culture
Genotypes
Cell line
Transgene insertion
Not detected (tmr)
tmr
35Spro-GFP-AtVam3p
35Spro-GFP-AtFim1
35Spro-GFP-tubulin
35Spro-GFP-AtRer1B
35Spro-NtTIP1;1-GFP
35Spro-CjSMT
35Spro-GFP-AtSYP31/35Spro-ST-mRFP
35Spro-GFP-AtSYP41/35Spro-ST-mRFP
35Spro-mRFP-AtSYP31
Protocol
PCR
Primer sets 《Primer sequences》
Cell line
Target
Forward
Reverse
Product size
CRA
tmr
35Spro_F3
GV7_R
Not detected (370 bp)
V208
tmr
35Spro_F3
GV7_R
370 bp
GV7
35Spro-GFP-AtVam3p
35Spro_F3
GV7_R
1.5 kbp
GV7
35Spro-GFP-AtVam3p
GFP_F
GV7_R
1.3 kbp
GF11
35Spro-GFP-AtFim1
35Spro_F3
GF11_R
2.0 kbp
GF11
35Spro-GFP-AtFim1
GFP_F
GF11_R
1.7 kbp
GT16
35Spro-GFP-tubulin
35Spro_F3
GT16_R
2.1 kbp
GT16
35Spro-GFP-tubulin
GFP_F
GT16_R
1.9 kbp
TBY2-AtRER1B
35Spro-GFP-AtRer1B
35Spro_F3
RER1B_R
1.4 kbp
TBY2-AtRER1B
35Spro-GFP-AtRer1B
GFP_494_F
RER1B_R
800 bp
TBY2-AtRER1B
35Spro-GFP-AtRer1B
GFP_F
RER1B_R
1.2 kbp
TBY2-AtRER1B
35Spro-GFP-AtRer1B
GFPS65T_F
RER1B_R1
565 bp
BY-TIPG
35Spro-NtTIP1;1-GFP
35Spro_F3
GFP_R
1.3 kbp
BY-TIPG
35Spro-NtTIP1;1-GFP
NtTIP1_F
GFP_R
1.0 kbp
156-S
35Spro-CjSMT
35Spro_F2
SMT_R
1.5 kbp
156-S
35Spro-CjSMT
35Spro_F2
NOSterR1
2.0 kbp
TBY2-31/ST
35Spro-GFP-AtSYP31
35Spro_F5
SYP31stop_R
1.8 kbp
TBY2-31/ST
35Spro-ST-mRFP
35Spro_F2
mRFPstop_R
1.1 kbp
TBY2-41/ST
35Spro-GFP-AtSYP41
35Spro_F5
SYP41stop_R
1.8 kbp
TBY2-41/ST
35Spro-ST-mRFP
35Spro_F2
mRFPstop_R
1.1 kbp
TBY2-R31
35Spro-mRFP-AtSYP31
35Spro_F5
SYP31stop_R
1.8 kbp
PCR reaction mixture
Component
10 µL reaction
TaKaRa Ex Taq™ Premix
5 µL
Forward primer (10 µM)
0.5 µL
Reverse primer (10 µM)
0.5 µL
Template DNA
1 µL
Sterile distilled water
3 µL
PCR reaction
Temperature
Time
Cycles
94°C
1 min
94°C
30 sec
35 cycles
55°C
30 sec
72°C
1 min/1 kbp
72°C
10 min
Check the size of the PCR product.
If necessary, check the DNA sequence of the PCR product.