=================
Primer sequences
=================

DNA barcoding
-------------

  .. csv-table::
     :header: "Primer name", "Direction", "Sequence"
     :widths: auto

     "13", "Forward", "ATGTCACCACAAACAGAGACTAAAGC"
     "18", "Reverse", "GCAGCAGCTAGTTCCGGGCTCCA"
     "2250", "Forward", "TTGGGAGTTCCTATCGTAATGCATG"
     "2356", "Reverse", "CATTCATAAACTGCTCTACCATAGT"
     "2384", "Reverse", "ACGTAAAGCTTTAGCTAGTACACGG"
     "2385", "Forward", "AAACTTTCCAGGGACCACCTCATGG"
     "atpF_f", "Forward", "ACTCGCACACACTCCCTTTCC"
     "atpH_r", "Reverse", "GCTTTTATGGAAGCTTTAACAAT"
     "Lj_atpF_seqR2", "Reverse", "CCCCCTTTTTGGGTTTTAGA"
     "matK_F", "Forward", "ACCCAGTCCATCTGGAAATCTTGGTTC"
     "matK_F1", "Forward", "TTACGATCTATTCATTCAATATTTCC"
     "matK_R", "Reverse", "CGTACAGTACTTTTGTGTTTACGAG"
     "matK_R1", "Reverse", "CAAATGGGTTTACTAATAGGATGCC"
     "psbK_f", "Forward", "TTAGCCTTTGTTTGGCAAG"
     "psbI_r", "Reverse", "AGAGTTTGAGAGTAAGCAT"
     "rbcL-R", "Reverse", "TGTCTTCGCGGTGGACTTGATTTTAC"

Transgene insertion
-------------------

  .. csv-table::
     :header: "Primer name", "Direction", "Sequence"
     :widths: auto

     "046D04_flankingseq_side", "Reverse", "AGAACATGCAGCCAGAGGTT"
     "046D04_RBside", "Forward", "TCATTTGTGGCAGGAAAACA"
     "2625", "Forward", "ATAATAACGCTGCGGACATCTACATTTT"
     "35Spro_F2", "Forward", "CAGTGGTCCCAAAGATGGAC"
     "35Spro_F3", "Forward", "ATTGATGTGATATCTCCACTGACGT"
     "35Spro_F5", "Forward", "TTCGCAAGACCCTTCCTCTA"
     "GF11_R", "Reverse", "CCGCTCAATGAGGAGA"
     "GFP_494_F", "Forward", "ACTTCAAGATCCGCCACAAC"
     "GFP_F", "Forward", "ACGTAAACGGCCACAAGTTC"
     "GFP_R", "Reverse", "GTCCTCCTTGAAGTCGATGC"
     "GFPS65T_F", "Forward", "CACATGAAGCAGCACGACTT"
     "GT16_R", "Reverse", "CACCTTCCTCCATACC"
     "GV7_R", "Reverse", "TCCTTGTGAAGTTGCA"
     "mRFPstop_R", "Reverse", "TAAGCTTAGGCGCCGGTGGAGT"
     "NOSter_R1", "Reverse", "GATCTAGTAACATAGATGACACC"
     "NtTIP1_F", "Forward", "CCTCTATAGCGCATGCCTTC"
     "pTiT37-F", "Forward", "gtagctcttgcccagcagac"
     "pTiT37-R", "Reverse", "ctgcttaactctggccttgg"
     "RER1B_R", "Reverse", "GCTGTTCGCAGAAGATTTCC"
     "RER1B_R1", "Reverse", "CCCTCCATGCCTCATGTACT"
     "SMT_R", "Reverse", "CTTAGGCCAAAACCCACAAA"
     "SYP31stop_R", "Reverse", "GTCGACTTAAGCCACAAAGAAGA"
     "SYP41stop_R", "Reverse", "CGTCGACTCACAAGAATATTTCC "

DNA sequencing
--------------

  .. csv-table::
     :header: "Primer name", "Direction", "Sequence"
     :widths: auto

     "145_F", "Forward", "AATGAGGACGATGACGATGAC"
     "145_R", "Reverse", "TCCAGTACCCCTTCTCAACA"
     "GCR_F2", "Forward", "CAGCYGGAAGGAGACAAATAG"
     "GCRseq_R1", "Reverse", "GGCATGGAACACCAAAGTCT"
     "gnom_At1g133980_F", "Forward", "TGAGTACACGCCTTTCTGGA"
     "gnom_At1g133980_R", "Reverse", "CAAGAGCAGCATCCTTGTTG"
     "PCRpsbALEFT", "Forward", ""
     "PCRpsbARIGHT", "Reverse", ""
     "Sp_NR_F6", "Forward", "TGATAACGTCACTCCTTCATACG"
     "Sp_NR_R1", "Reverse", "ATTGCCATTCCATGTGAAAA"
     "Spi8_F", "Forward", "CCTGTCGAGGGTGATTTTGT"
     "Spi8_R", "Reverse", "CCAAAGCCAAAGAGATGTGC"
     "tom1-2_F", "Forward", "TGTTGCAATTTCTGACCTTTTG"
     "tom1-2_R", "Reverse", "GCAAAGAGCCTGCATTACCT"
     "Tom3-1_F", "Forward", "AAGAGGTGATTCAAATCCAGAGA"
     "Tom3-1_R", "Reverse", "TGAGCCAAATCAATCAATCAA"

Fragment analysis
-----------------

  +------------+---------+-----------------------------------------+
  |Primer name |Direction|Sequence                                 |
  +============+=========+=========================================+
  |3483        |Forward  |gtgtcttAAAGAGGAAACCGTGTCTCG              |
  +------------+---------+-----------------------------------------+
  |3484        |Reverse  |cccccccccccccctgaGAGATAACCAAACACGCAACAA  |
  +------------+---------+-----------------------------------------+
  |3493        |Forward  |gtgtcttTCGTGCATGCTTTTCTTGAC              |
  +------------+---------+-----------------------------------------+
  |3494        |Reverse  |cccccccccccccctgaCCACCTAGTCTCACCCGTTT    |
  +------------+---------+-----------------------------------------+
  |3495        |Forward  |gtgtcttTCCCCTTTTGCTTCTTACCC              |
  +------------+---------+-----------------------------------------+
  |3496        |Reverse  |cccccccccccccctgaCAACAGATTCCTTGCTTGATGA  |
  +------------+---------+-----------------------------------------+
  |3497        |Forward  |gtgtcttGAGACGTACCAGGACATTGTTGT           |
  +------------+---------+-----------------------------------------+
  |3498        |Reverse  |cccccccccccccctgaGCATACTGAATGTCATGAAAGTGG|
  +------------+---------+-----------------------------------------+
  |uni-FAM [#]_|         |cccccccccccccctga                        |
  +------------+---------+-----------------------------------------+

  .. [#] Fluorescent labeled primer at 5′ end with 6-FAM™