Lotus japonicus cell lines
Plant cell lines
Genotypes
Protocols
DNA barcoding — rbcL
DNA barcoding — atpF–atpH
PCR
Primer set 《Primer sequences》
Target
Forward primer
Reverse primer
Product size
atpF–atpH
atpF_f
atpH_r
800 bp
PCR reaction mixture
Component
10 µL reaction
TaKaRa Ex Taq™ Premix
5 µL
Forward primer (100 µM)
0.5 µL
Reverse primer (100 µM)
0.5 µL
Template DNA
1 µL
Sterile distilled water
3 µL
PCR reaction
Temperature
Time
Cycles
95°C
1 min
94°C
30 sec
35 cycles
52°C
30 sec
72°C
1 min
72°C
10 min
Check the DNA sequence of the PCR product.
NCBI BLAST search
Compare the DNA sequence with that of previous years.